Coding

Part:BBa_K3352000:Design

Designed by: Joyce Ting   Group: iGEM20_TAS_Taipei   (2020-10-17)


SplintR Ligase with His-Tag and GS Linker Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 790
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We attached 6x histidine tag upstream of the SplintR Ligase sequence for purification purposes followed by a glycine-serine linker (GS linker).


Source

SplintR ligase protein found in Paramecium bursaria Chlorella virus

References